Effect of Naphthalene acetic acid and date palm pollen extraction on gene expression on fruit of Hillawi date palm cultivar during growth and ripening

Abstract

This study was carried out on fruit of Hillawi date palm cultivar which pollinated by Khukri Adi and
Ghunami Akhder cultivars to study the effect of NAA 25 ppm and pollen extraction of Khukri Adi and
Ghunami Akhder 2g/L which were sprayed at 30 and 45 day after pollination on gene expression by
studying the changes in protein pattern during fruit development and ripening by gel electrophoreses
method with sodium dodecyle sulfate (SDS-PAGE). The result showed there were many protein band
which were different in molecular weight and numbers on the gel with changes on fruit growth stages and
treatments, These bands of protein were similar in early stage of growth but different in the period of
13,15 weeks after pollination, this difference may be correlated with ripening date. Our results showed
that we could consider the protein pattern as gene expression markers for fruit and linking them with a
different growth and ripening stage and explanation of metaxenia phenomenon.