In vitro Effect of Activated Charcoal and microcutting type on microtuberization of potato CV. Desiree
DOI:
https://doi.org/10.24126/jobrc.2007.1.1.10Abstract
Tips of sprouts (0.3–0.5 mm) of Desiree potato sterilized and cultured on proliferation media. The cultures were incubated for 35 days at 16 hours photoperiod at 25 ± 2 º C under 1000 loux light intensity. In vitro – proliferated shoot were cut at 1-2 cm into three types of microcuttings (i.e. apical, subapical and basal) and cultured onto MS media supplimented with 10% sucrose for microtuberization. Activated Charcoal treatments 0.0, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 gm /l were added to the microtuberization media .The weight, size and eyes number cultured of microtubers were significantly increased when basal microcuttings cultured on microtuberization media supplimented with 3gm /l charcoal while 2gm/l charcoal with the subapical cuttings resulted in high proliferation rate
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