Cytogenetic Analysis of Goat Blood Lymphocytes cultured in Vitro

Abstract

The aim of this work is to determine the duration of goat cell cycling in vitro.Goat peripheral blood lymphocytes were grown in RPMI-1640 medium containing bromodeoxyuridine (BrdU 10 μg/ml) for 72 h. Blastogenic index (BI), mitotic index (MI), cell cycle progression (CCP) and sister chromatid exchanges (SCE) were determined. Cultured lymphocytes from, whole blood or from leukocyte rich plasma in RPMI-1640 medium containing BrdU showed little differences in BI, MI, but significant differences were seen in cell cycle progression. BI, MI, and CCP from different goat breed were compared. Also, the percentage of lymphocyte blastogenesis, mitoses and cell cycle progression from goat, were compared to those from sheep, and human whichgrown under similar conditions. On successive incubation periods, the cell cycle duration of blood lymphocytes was determined through the mitotic activity. The cells reached first, second and third mitoses after 25, 40 and 48 h, post incubation respectively. Sub culturing of growing lymphocytes was performed from 3 to 45 days to obtain a lymphoblastoid cells. The characterization of their differentiation is required Establishment of goat blood lymphocyte culture will help in gene marker’s detection in their somatic cells.