Production of Procyanidin compound in seeds , callus and in regenerated plants from vitis vinifera L.

Abstract

This experiment was conducted in Science and Agriculture College labs, Kufa University, on 2008 to describe methods for extraction and purification of Procyanidin compound from each of grape seeds mother plant(Vitis vinifera L.) ,callus induced from Axillary buds by using MS medium supplemented with 2,4-D at concentration of (1,2,3)mg/l and from leaves of regenerated from callus by using MS medium supplemented with ( IAA )at concentration of 2mg/l and (BA) benzyl adenine at concentration of 0.2 mg/l and from cocoas powdered (control) as well these Procyanidin. The content of Procyanidin were done by using HPLC (high performance liquid chromatography) and compare of the quantitatively of Procyanidin with these seeds in mother plant and callus tissue. This study include alcohol extraction by using mixture of ethanol, methanol, D.W and HCL. The result revealed that, the superiority of Procyanidin in seeds content than the content in callus and regenerated plant and increase the content of these compound in the leaves of regenerated plant with the content in callus tissue.