In vitro propagation of some tomato (Lycopersicon esculentum Mill.) hybrids

Authors

  • Kadhum Muhammed Ibrahem
  • Hamza Musa Kadhum
  • Ali Abadi Manea

DOI:

https://doi.org/10.24126/jobrc.2007.1.1.14

Abstract

Seeds of four tomato hybrids Ginan, Nirtia, Speedy and Rola were cultured on aseptic germination medium after surface sterilization with NaOCl. Shoot tips 1cm length were dissected and grown on MS medium supplemented with Benzyl adenine (BA) and Indole acetic acid (IAA) for multiplication. Callus was induced on hypocotyls cultured on MS medium supplemented with BA under light intensity of 1000 lux and 25 0C. Results showed that NaOCl at 4% completely disinfected seeds and resulted in pathogen free seedlings. Results also showed that a concentration of 4 mg/l of BA was superior in increasing callus fresh and dry weights. Shoot multiplication was achieved on the same medium supplemented with 2 and 0.8 mg/l of BA and IAA respectively.

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Published

2007-01-01

How to Cite

Ibrahem, K. M. ., Kadhum, H. M. ., & Manea, A. A. . (2007). In vitro propagation of some tomato (Lycopersicon esculentum Mill.) hybrids. Journal of Biotechnology Research Center, 1(1), 52–60. https://doi.org/10.24126/jobrc.2007.1.1.14

Issue

Vol. 1 No. 1 (2007): Issue 1

Section

Research articles

License

This is an Open Access article distributed under the terms of the creative commons Attribution (CC BY) 4.0 license which permits unrestricted use, distribution, and reproduction in any medium or format, and to alter, transform, or build upon the material, including for commercial use, providing the original author is credited.