Production an immuno kit for identification two strains of Ralstonia solancearum (smith) by ELISA which caused a bacterial wilt on tomato in Iraq

Abstract

Ralstonia solancearum is one of most important tomato pathogen which annually causes considerable yield loss. Strains of these pathogenic bacteria were identified based on both phenotypic and differentiation media on Tetrazolium Chloride Agar (TZC) and the hypersensitivity tests on leaves of tobacco cultivar xanthi. Many biochemical adapted tests were carried out for identification of both the genus and species of the bacteria. Two strains R1 and R3 were identified. Antiserum of each strain was obtained following the injection of pure cell suspension of each strain in lab. Rabbit. Immunoglobulin (IgG) was purified and conjugated with alkaline phosphates and used as an identification kit for each strain by ELISA test .The reading of ELISA reader at 405 nanometer when testing plant extract infected by R1 strain with its antiserum were 1.14 while it was 1.10 for R3 strain, ELISA test were also carried out for plant extract infected by R1 strain and the antiserum of R3 strain, ELISA test were carried out too for plant infected by R3 strain and antiserum of R1 strain the reading of ELISA reader were 0.90, 0.92 respectively. The result of ELISA test was negative when using the normal serum and carbonate buffer as antiserum. The result of this test is clear evidence showed the high specify of the antiserum which produced in this study.