Effect of Sucrose and Naphthalene acetic acid Concentrations on Rooting of Sugarcane In vitro

Abstract

hoots of two sugarcane genotypes (Co.J.64, Co.J.86) were rooted in vitro on MS medium supplemented with different concentration of sucrose (0, 30, 50, 60, 70) gm/l or NAA (0, 2.5, 5.0, 7.5, 10.0) mg/l in separate experiments. All cultures were incubated at 25±2C◦with 16 h/d light (1000 lux) for 60 days. Data of roots per plant, length, dry and fresh weights were taken after 8 weeks. Results showed that 70g/l of sucrose gave higher mean in length and fresh weight of roots reached 1.70 cm /plant, 19.80 mg/root, respectively, while 60g/l sucrose gave higher mean in number and dry weight reached 9.95 root, 4.60mg, respectively. NAA concentrations were significantly effected in root length reached 1.23 cm/plant at 5 mg/l NAA while a significant effect in root number, fresh and dry weight. The genotype Co.J.64 was the best in number of roots in sucrose experiment, while genotype Co.J.86 surpassed in number of root, fresh and dry weight in NAA experiment.