Determination of the optimum conditions for Gelatinase production from local isolate Enterococcus faecalis B91

Abstract

A total of 70 isolates belonged to Enterococcus faecalis collected from different clinical sources : (29 urine sample, 22 stool sample, 9 blood sample, 1 vagina sample, 2 sputum sample, 1 pleural fluid sample, 1 peritoneal fluid sample and 5 wounds sample). Seven isolates were able to produce gelatinase since they were able to liquefied the gelatin medium and clear zone were observed around the bacterial growth after floating the plate with freizer's reagent. Enterococcus faecalis B91 was higher producer of gelatinase in submerged culture by using Yeast extract gelatin Medium. Optimization study revealed that fructose was the best carbon source at 2.5% and 1% gelatin as inducible source to production the enzyme and inoculum size 1x109 cfu/ml at initial pH7, incubated for 24 hours at 40°C by using shaking incubator at 150 rpm.