Extraction of Catechin from green tea plants Camellia sinensis

Authors

  • Nazar E. Nasser
  • Yasir A. J. Al-abdli
  • Essam F. A. Al-Jumaily

DOI:

https://doi.org/10.24126/jobrc.2011.5.3.173

Keywords:

Green tea, Catechin, HPLC, TLC

Abstract

Catechin was extracted from green tea Camellia sinensis by using liquid-liquid chromatography. Extraction was done using hot water and then fractionation of the extract in two-stages first mixed with chloroform; the aqueous layer was taken and the second step mixed with ethyl acetate to remove all the apolar compounds and dark brown colour substance was obtained nonviscous and this was supposed to be catechin compound. Catechin has been diagnosed by using chemical reagent; thin layer chromatography (TLC) and the relative front (Rf) was 0.92 when using the mobile phase acetic acid: chloroform (9:1). The melting point has reached 120oC. The catechin was conducted by the use of high-performance liquid chromatography (HPLC) and was diagnosed by FTIR spectrum.

Downloads

Published

2011-12-01

How to Cite

Nasser, N. E. ., Al-abdli, Y. A. J., & Al-Jumaily, E. F. A. . (2011). Extraction of Catechin from green tea plants Camellia sinensis. Journal of Biotechnology Research Center (JOBRC), 5(3), 9–14. https://doi.org/10.24126/jobrc.2011.5.3.173

Issue

Vol. 5 No. 3 (2011): Issue 3

Section

Research articles

License

This is an Open Access article distributed under the terms of the creative commons Attribution (CC BY) 4.0 license which permits unrestricted use, distribution, and reproduction in any medium or format, and to alter, transform, or build upon the material, including for commercial use, providing the original author is credited.