Effecienty of Immobilization technique of Bacillus Sublilis Asperginase producer

Abstract

wenty Bacillus isolated were obtained from different sample food and water. Bacillus B1 isolated was the highest asparaginase producer, it was identified as a strain of B. subtilis.The highest production of asparaginase was observed when mineral salt medium containing 0.3% asparagen, pH 8 and incubated at 400c for 24 hrs. B. subtilis B1 cells were immobilized by entrapment methods (calcium alginate and agar), and by adsorption on solid surface such as sawdust and cotton. The result showed that the immobilized cells by adsorption on sawdust was the best, the immobilized cell retained 88% of asparginase activity after 48h while free cell retained 65%. Cells immobilized by adsorption on sawdust was incubated at different temperatures (37-60)0c for 12 min. and at different pH (4-10) for 120 min. the result showed that the immobilized cell had 78% remaining activity at 37c while the free cells were 58%, and retaining activity was 70% at pH=7 while free cells were 52%.