Effect of bioactive compounds in ginger extract on cancer cell lines In vitro.

Abstract

his study was designed to detect the active compounds found in ethanolic crude extract of Zingeber officinale rhizome. Chemical detection of extract appeared that the ethanolic extract contain flavonoid, saponin, glycosids, phenols, resins, volatile oils, tannins, terpins and steroids while alkaloids and cumarines gave negative results. Different concentrations were prepared from ethanolic extract starting from (1-1000) µg/ml to evaluate the cytotoxic effect of ethanolic extract on two malignant cell lines, human laryngeal carcinoma (HEP-2) cell line and murine mammary adenocarcinoma (AMN-3) cell line, exposure periods of cell lines were measured at (24, 48, 72)hr in a microtitration plate under complete sterile conditions. Results showed that, Ethanolic crude extract exhibited time-dependent cytotoxic effect of all concentrations after exposure for 24hr on cancer and transformed cells lines, and the 100 µg/ml gave the highest inhibition rate for AMN-3 86.2% after 24hr of exposure time and the cytotoxic effect of the extract started at lowest concentrations on HEP-2 cell line after 48hr of exposure reached which the best concentration 600 µg/ml gave the highest inhibition cell growth 73.4% after 48hr of exposure time.