Using plant tissue culture technique to evaluate of three genotypes (Triticum aestivum L.) to salinity tolerance.

Abstract

he effect of five levels of sodium chloride (0.0, 0.5, 1.0, 1.5, 2) % on callus initiated from immature embryos of three genotypes of Triticum aestivum L. (I.E.Tamose 2, Rabeia, genotype 20) were investigated. Callus fresh and dry weight, cell contents of proline, carbohydrate as well as Na, Cl, K and Ca ions were used as parameters to determine the effect of NaCl on callus culture. The results showed Significant differences between genotypes in the most parameters studies significant reduction in callus fresh and dry weight as well as callus content of K, Ca ions and carbohydrate with NaCl concentration increased in the medium . On the other hand proline concentration Na and Cl ions were significant increased with NaCl concentration increased in the culture medium. Significant interactions were recorded between genotypes and salt concentration in their parameters.