Detecting some factors affecting Salmonella typhimurium adhesion and inhibition by Saccharomyces boulardii

Abstract

ntibiotic sensitivity of Salmonella isolates was investigated, the isolates were sensitive to some antibiotics such as chloramphenicol, imipenem and most of them showed resistance to amoxicillin and naldixic acid. The hydrophobicity of Salmonella was investigated by applying BATH test (Bacterial Adherence To Hydrocarbons) using xylene. The range was between (21-90) %, the results showed that five isolates were hydrophobic including sal10 isolate. The surface layer proteins (SLP)of sal10 showed the ability to act as agglutinins by applying haemagglutination test with and without 0.5% mannose, also the bacteria seemed to possess pili -type1 because of its’ ability to agglutinate with human blood cells and yeast cells. The agglutination of the bacteria was inhibited in the presence of mannose.The isolate sal10 was adhesive to buccal epithelial cells in an average 52 cell/epithelial cell, while this average decreased to reach 24 cell/ epithelial cell in the presence of Saccharomyces boulardii which was able to inhibit 54% of the bacterial adherance.