Optimum conditions for isoamylase production by Pseudomonas sp.

Authors

  • Hameed M. Jasim
  • Haneen S. Abdul-Wahab

DOI:

https://doi.org/10.24126/jobrc.2013.7.1.232

Abstract

Different nutritional and cultural factors were studied to determine the optimum conditions for isoamylase production by Pseudomonas sp. in a batch culture of the production medium. These factors include carbon, nitrogen and phosphate sources and their concentrations, temperature and pH. Results showed that the optimum conditions for isoamylase production by Pseudomonas sp. were achieved when the production medium was supplemented with maltose 1%, peptone 0.4%, and K2HPO4 0.4% as a carbon, nitrogen, and phosphate sources respectively, at initial medium pH 6, and incubation at 28°C for 24 hours. Under these conditions isoamylase productivity reaches the maximum, at which enzyme specific activity was 0.85 U/mg proteins.

Downloads

Published

2013-01-01

How to Cite

Jasim, H. M. ., & Abdul-Wahab, H. S. . (2013). Optimum conditions for isoamylase production by Pseudomonas sp. Journal of Biotechnology Research Center (JOBRC), 7(1), 3–10. https://doi.org/10.24126/jobrc.2013.7.1.232

Issue

Vol. 7 No. 1 (2013): Issue 1

Section

Research articles

License

This is an Open Access article distributed under the terms of the creative commons Attribution (CC BY) 4.0 license which permits unrestricted use, distribution, and reproduction in any medium or format, and to alter, transform, or build upon the material, including for commercial use, providing the original author is credited.