Callus Cultures derived from electrotreated cell suspension of Dianthus caryophyllus L. embedded in agar drops express their totipotency

Authors

  • Sahla Mohammed Zedan
  • Djamela Haza’a rasheed

DOI:

https://doi.org/10.24126/jobrc.2013.7.2.264

Keywords:

المعلقات الخلوية، القرنفل، طاقتها الكامنة

Abstract

Callus cultures were produced from leaf, excised from axenic carnation, Dianthus caryophyllus L. seedling, on agar-solidified MS medium supplemented with 0.5 mgl-1 of 2,4-D and 0.1 mgl-1 of BA. Cell suspension was initiated in liquid MS medium containing the above growth regulators. This suspension reported the density of 143 × 103 cell /ml and its viability was ranged between 41-90%. Samples of cell suspension represents seven different densities were exposed to three electrotreatment 300v/50 msec, 500v/100msec. and 700v/150 msec. These electrotreated samples were cultured by embedding in to agar drops in solid- liquid cultures. Callus derived from cell suspension expressed their totipotency forming carnation plants.

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Published

2013-06-01

How to Cite

Zedan, S. M. ., & rasheed, D. H. . (2013). Callus Cultures derived from electrotreated cell suspension of Dianthus caryophyllus L. embedded in agar drops express their totipotency. Journal of Biotechnology Research Center, 7(2), 32–38. https://doi.org/10.24126/jobrc.2013.7.2.264

Issue

Section

Research articles