Detection of plasmid DNA profile in bacterial and fungal isolates from catheterized patients and its relation with antibiotic susceptibility

Abstract

Study the resistance of bacterial and fungal isolates which identified previously towards different antibiotics that have been recommended for treating those patients. It was found that higher percentage of resistance to penicillin, gentamycin, tetracyclin, chlormphenicol, and trimethoprim- sulfmethazxol then moderate resistance to ampcillin, nalidixic acid, cephotaxime, streptomycin, cephalexin, rifampicin and less resistance to ciprofloxacin that represent the most effective antibiotic against uropathogenic bacteria. Ketazole antibiotic was used against yeasts, which the minimum inhibitory concentration of ketazole against 83% of Candida. albicans isolates was 128 μg/ml, the rest of the isolates was 64 μg/ml. Isolation of bacterial and yeasts DNA (chromosome + plasmids), results of DNA isolation showed that bacterial isolates (E.coli (RS1), proteus. mirabilis (RS2), klebsiella. pneumonia (RS3) and Pseudomonas. aeruginosa (RS4) have chromosome and plasmids vary in numbers and size according to isolate. While yeast (C13) isolate have only chromosome without plasmid.A result of curring shows that:-Resistance to ampicillin, tetracycline, gentamycin, pencillin for all bacterial isolates carried on plasmid.- Resistance to cephalexin by RS1 isolate, resistance to cephotaxim by RS3 isolate and resistance to chlormphnicol by RS4 isolate carried on plasmid, while resistance to other antibiotics were carried on chromosome.