Callus induction from Nerium oleander leaves and qualitative determination of some active compounds in vivo and in vitro

Abstract

Callus induction of Nerium oleander young leaves explants was studied using Murashige & Skooge (MS) Medium supplemented with 2,4-dichlorophenoxy acetic acid( 2,4-D 0.0, 0.5, 1.0, 2.0) mg/l with Benzyle adinine (BA0.0, 0.5 or 1.0, mg/l). comparative study was made for qualitative determination of some active compounds between the initiated callus and leaves of fieled plant (intact plant). Results showed that 2,4-D at 1.0 or 2.0 mg/l with BA at 0.5 or 1.0, mg/l were the best for callus induction that gave the highest fresh and dry weight. Qualitative detection showed that (Alkaloids, Flavonoids, Terpenoids, Tanins, Saponins, and Cardiac Glycosides) the main active compounds were found in callus and leaves of field-grown plant.