Study the effect of aqueous extracts of Pimpinella anisum , Foeniculum vulgara and agroup of different medical herbs on growth of bacteria ( Staphylococcus aureus) isolated from Sputum of bronchiolitis patients .

Abstract

This study was conducted to determine the inhibitory dose of the hot and cold aqueous extracts of Pimpinella anisum (PA) , Foeniculum vulgara (FV) , and agroup of different medical herbs on growth of bacteria , Staphylococcus aureus isolated from the sputum of bronchiolitis patients . Minimum Inhibitory concentration (MIC) method was used to test the sensitivity of the bacteria toward the above extract . The hot aqueous extract of (PA)in concentration of 23.8 and 28mg/ ml gave a strong inhibitory ability on the growth of the bacteria at MIC 0.01 mg/ ml compared with the highest concentration of 47.6 mg/ml which did not inhibit the bacterial growth at MIC 0.02 mg/ml . The cold aqueous extract of PA at 125 mg /ml concentration also did not inhibit the bacterial growth up to MIC 0.12 mg / ml . This study also revealed that a concentration of 17.12 mg / ml of hot aqueous extract of FV seeds had no inhibitory effect on growth up to MIC 0.008 mg / ml . The combined hot aqueous extract of PA and FV seeds in a ratio of 1gm to 0.5 gm respectively an 1gm to 1 gm (24and 40.32mg / ml respectively ) also did not have any inhibitory effect of bacterial growth up to MIC 0.002 and 0.004 mg / ml, respectively . The results of this experiment also revealed that hot aqueous extract of similar quantities and concentrations ( 17.28 and 31.74 mg /ml ) of a mixture of several medical herbs like ( PA , FV , Anthemis nobilis , Thymus bovei ,Gum Arabic, Nigella sativa , Althaea , and Cydonia vulgaris ) did not show any inhibitory action on bacterial growth up to MIC of 0.004 and 0.007 mg / ml . It could be concluded that the inhibitory ability of the hot aqueous extract of PA seeds is the best compared to the other extracts used . The inhibition of bacterial growth may need a carefully selected medical herbs in different concentrations and combination .