Detection of Perforin in suspension of leukemia lymphocyte culture

Authors

  • Najwa Sh. Ahmed
  • Amal G.S. Fayyad
  • Wan Mohter Yusoff
  • Subhi Jawad Hamza
  • Abdul Hussen Al-Faisal
  • Adeeb A. AL-Shami

DOI:

https://doi.org/10.24126/jobrc.2014.8.1.293

Keywords:

Perforin, leukemia lymphocyte

Abstract

This work aimed to study the concentrations and hemolytic assay of perforin in serum and culture suspension of lymphocyte for acute lymphocyte leukemia ALL; chronic lymphocyte leukemia CLL, and control. To achieve this goal, blood samples were collected from 20 leukemic cases 10 ALL and 10 CLL, and isolation of lymphocyte done then proliferation of leukemia lymphocyte in culture for the detection of perforin concentration by ELISA kit and hemolytic activity of perforin in suspension lymphocyte culture and serum were impacted. The results, showed significant increase in the level of concentrations of perforin in both CLL and ALL as compared with the control, however, showed significant lower in level of hemolytic activity of perforin in these groups as compared to the control. The conclusion is that expression of perforin is strongly associated with leukemia patient in Iraqi population.

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Published

2014-01-01

How to Cite

Ahmed, N. S. ., Fayyad, A. G. ., Yusoff, W. M. ., Hamza, S. J. ., Al-Faisal, A. H. ., & AL-Shami, A. A. . (2014). Detection of Perforin in suspension of leukemia lymphocyte culture. Journal of Biotechnology Research Center, 8(1), 20–24. https://doi.org/10.24126/jobrc.2014.8.1.293

Issue

Vol. 8 No. 1 (2014): Issue 1

Section

Research articles

License

This is an Open Access article distributed under the terms of the creative commons Attribution (CC BY) 4.0 license which permits unrestricted use, distribution, and reproduction in any medium or format, and to alter, transform, or build upon the material, including for commercial use, providing the original author is credited.