Detection of Perforin in suspension of leukemia lymphocyte culture

Abstract

This work aimed to study the concentrations and hemolytic assay of perforin in serum and culture suspension of lymphocyte for acute lymphocyte leukemia ALL; chronic lymphocyte leukemia CLL, and control. To achieve this goal, blood samples were collected from 20 leukemic cases 10 ALL and 10 CLL, and isolation of lymphocyte done then proliferation of leukemia lymphocyte in culture for the detection of perforin concentration by ELISA kit and hemolytic activity of perforin in suspension lymphocyte culture and serum were impacted. The results, showed significant increase in the level of concentrations of perforin in both CLL and ALL as compared with the control, however, showed significant lower in level of hemolytic activity of perforin in these groups as compared to the control. The conclusion is that expression of perforin is strongly associated with leukemia patient in Iraqi population.