Cytogenetic Studies on Goat Blood Lymphocytes:Cell Cycling
DOI:
https://doi.org/10.24126/jobrc.2009.3.1.32Abstract
Peripheral blood lymphocytes from goats (local breed) were cultivated in RPMI-1640 medium containing 15µg/ml of BudR 20 µg/ml of PHA for different times (12, 24, 36, 48, 60, 72 and 96( hrs. to determination the cell cycle duration. Blastogenesis was appeared post first 12hr of cultivation followed by first mitoses post 24 hrs. of culture initiation. The second and third cell cycling lasted 22 and 21 hrs, respectively. Effects of 6-thioguanine, methotrexate , colchicine and tamoxifen on cell cycle progression were investigated. Goat cells were found to be resistant to tamoxifen and MTX and sensitive to 6 TG and colchicine, which could be use as genetic markers to chick cellular genome integrity. Priming of goat blood lymphocytes was achieved by treating the blood with PHA for 24hr. Such treatment increased the in vitro growing period of derived lymphoblasts with short cycling time. However, PHA was found to be a promoting factor for initiation of blastogenesis and cell divisions in goat blood lymphoblasts. These techniques: Genetic markers, cytogenetic analysis cell cycling and lymphoblast explantation are crucial processes for nuclear transplantation processes.
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This is an Open Access article distributed under the terms of the creative commons Attribution (CC BY) 4.0 license which permits unrestricted use, distribution, and reproduction in any medium or format, and to alter, transform, or build upon the material, including for commercial use, providing the original author is credited.
