Extraction and partial Purification of ipopolysaccaride (LPS) from E. coli O157:H7 ioslate

Ashwak B.  J. Al-Hashimi; Essam F. A.  Al-Jumaily; Amina N.  Al-Thiwini; Hitham A.  Al-Omari

doi:10.24126/jobrc.2009.3.1.34

Extraction and partial Purification of ipopolysaccaride (LPS) from E. coli O157:H7 ioslate

Authors

Ashwak B. J. Al-Hashimi
Essam F. A. Al-Jumaily
Amina N. Al-Thiwini
Hitham A. Al-Omari

DOI:

https://doi.org/10.24126/jobrc.2009.3.1.34

Abstract

Lipopolysaccharide (LPS) was partialy purified from E. coli O157:H7 local isolate by Sepharose – 4B gel filtration chromatography, after extraction by phenol-chloroform –petroleum ether mixture. The results show the presence of two peaks of proteins; while one peak of carbohydrates when tested at wave length 490nm. Nucleic acids were not found in the sample after partial purification. Electrophoresis pattern shows one large band with a molecular weight of 69000 daltons.

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Published

2009-01-01

How to Cite

J. Al-Hashimi, A. B. ., Al-Jumaily, E. F. A. ., Al-Thiwini, A. N. ., & Al-Omari, H. A. . (2009). Extraction and partial Purification of ipopolysaccaride (LPS) from E. coli O157:H7 ioslate. Journal of Biotechnology Research Center (JOBRC), 3(1), 10–14. https://doi.org/10.24126/jobrc.2009.3.1.34

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Issue

Vol. 3 No. 1 (2009): Issue 1

Section

Research articles

License

This is an Open Access article distributed under the terms of the creative commons Attribution (CC BY) 4.0 license which permits unrestricted use, distribution, and reproduction in any medium or format, and to alter, transform, or build upon the material, including for commercial use, providing the original author is credited.