In vitro propagation of Narcissus tazetta L. bulbs

Abstract

In vitro technique was used to propagate Narcissus tazetta L. Two approaches were used. The first explants of basal discs and scale leaves from bulbs were cultured in MS medium containing different concentrations of BA and NAA. Cultures were incubated in light and dark. Results showed that a significant increasing in shoot numbers and lengths in both types lf explants. Treatments including; light + 7.0 mg / L BA + 0.0 mg / L NAA (15.8 shoots) and light + 3.0 mg / L BA + 1.0 mg/L NAA (9.04 cm) were significant for the basal disc. while the treatments: light + 3.0 mg / L BA + 0.0 mg /L NAA (12.5 shoots ) and light + 1.0 mg / L BA + 0.0 mg / L NAA (5.02 cm) were the best for scale leaves. Callus was induced largely in explants cultured in dark and high concentrations of NAA. The second experiment: shoots from above were cultured in MS medium containing sucrose 3 or 6% with IBA 0.5, 1.0 , 1.5 mg / L for bulb lets formation. The highest numbers of bulb lets 3.2, 3.4 were observed in: 3% sucrose + 1.5 mg / L IBA and 6% sucrose + 1.0 mg / L IBA respective. On the other hand, the bulb let diameter 7.42 mm and weight 320.4 mg were observed in: 3% sucrose + 1.5 mg/ L IBA and 3% sucrose + 0.5 mg / L IBA respectively. The formed bulb lets were transferred to peat moss plus river soil media, which then successfully grown and gave vegetative shoots in the percent 98%. Abbreviations: BA(6-benzyl adenine) ; NAA(α-naphthalene acetic acid ); IBA ( Indole – butyric acid ); MS ( Murashige and Skoog medium).