Effect of electric current and ultra violet of elements and protein in shoot of three Hybrids of Cucumis sativus L. In vitro

Abstract

Three hybrids of American cucumber were studied, they are Lot number EM285 labeled
number 1, Hybrid tocaeen F1 labeled number 2, Hybrid super green F1 labeled number 3. The
seeds of these hybrids were exposure to four frequencies of electric current 0,1,2 and 2.5 Am for
5 minutes and 4 periods of ultra violate rays UV at 254 nm. For 0,30,60 and 90 second. The
sterilized seeds were cultured in MS medium. After 3 weeks apical meristems of seedling were
excise and cultured in new MS medium and of 5 weeks shoot systems were taken to study the
element content nitrogen, phosphorous, potassium, calcium and magnesium in addition to
estimate protein percentage. The results showed that hybrid 3 had a significant increase of
element contents and protein percentage compared with hybrid1 and 2.The interactions between
hybrid3 and 90 sec. UV exposure showed the highest content of calcium element reached 2.73
mg.gm-1 with an increase of 184.37%compared with the control. The interaction between 2.5 Am
and 90 sec. UV exposure showed the highest rate of protein reached 15.5%with significant
differences of all interactions.