Genotyping of High-risk Human Papilloma virus (HPV) among Iraqi women in Baghdad by Multiplex PCR

Abstract

Human papilloma virus (HPV) infection is a causative factor for cervical cancer. Early detection
risk HPV types might help to identify women at high risk of cervical cancer. The aim of of high
HPV infection in population of rgir hgih determine the occurrence of the present study was to
Iraqi women in Baghdad by using Multiplex PCR determine the percentage and genotyping of
Human Papilloma Virus and to put the best prevention and control program in Iraqi women.
Study started at January 2009 to March 2010, cervical samples were collected from 856 women
aged 16–70. HPV DNA amplification was performed using HPV High Risk Typing PCR Kit test
for qualitative detection and genotyping of HPV types 16, 18, 31, 33, 35, 39, 45, 52, 56, 58, 59, 66 in
HPV was detected in 106 ( 12,38% ) of the study population, with a range of the cervical swabs.
16-70 years age groups. Results showed that the overall HPV prevalence twelve genotypes were
identified, including HPV-33 (18.60%), HPV-35 (18.60%), HPV-56 (18.60%) ,HPV-39(10.85%),
HPV-52 (10.08%), HPV-18 (7.75%), HPV-16 (4.65%), HPV-59 (4.65%), HPV-58(2.32%), HPV-31
(1.55%), HPV-45(1.55% ) and HPV-66( 0.77%). Of 856, 218 women was also tested by pap smear
ith normal cytology was 198 ( 90.83%), 24(12.12%) of them were HPV positive, those with w,
abnormal cytology was 20 (9.17 %), 5( 25%)of them was HPV positive. In this study unlike other
epidemiological studies, HPV33,35,56 was the most frequent type (55.8%) in Baghdad, followed
by HPV39, HPV52, HPV18, HPV16.