Efficient Regeneration of Carrot (Daucus carota L.) plants from cell suspension derived-callus

Abstract

This study succeeded in establishment carrot cell suspension cultures from stem callus in liquid
MS medium containing 1.0 mg L-1
of each NAA and BA. Its density approach 3.4 ×105
cell ml-1
at
the third day of culture. These cells ,continued, when embedded in agar drop using Multiple
Drop Array (MDA) technique, in division and forming cellular colonies which producing
numerous callus primordia that developed to callus cultures. When transferred to the solid
differentiation medium (MS+ 1.0 mg L-1 NAA+ 1.5 mg L-1 BA), two hundreds and seventy-three
shoots produced. They readily rooted in agar-solidified MSO medium after three weeks and
adapted in conditions of culture room. They were not transferred to field due to the unfavorable
environmental conditions at that time. Cell suspension-derived callus tissues contained 82.62 μg
anthocyanin gm-1 of callus fresh weight, and 0.555 mg beta-carotene 100 gm-1
of callus fresh
weight in the third period of extraction compared with their quantity calculated in stems callus
which recorded 52.4 μg gm-1
and 1.988 mg gm-1
respectively.