Antimicrobial Activity of Ethanolic and Watery Extracts to Fruit of Berberis vulagaris on growth of some species of pathogenic bacteria

Abstract

This study was done to test the activity of watery and alcohol extract of the fruit of Berberis
vulagaris against of two species of gram positive bacteria Bacillus cereus and Staphylococcus
aureus also ,two species of negative gram bacteria E. coli and Pseudomonas aeruginosa by agar
well diffusion method, the results showed that the alcohol extract of the Berberis vulagaris with
high activity against gram positive and the effect was increase with the increasing of the
concentration. While the watery and alcohol extract has a high effect on gram negative bacteria
which effect only with high concentration, while there is no effect in low concentration and the
results showed that the alcohol extract of the Berberis vulagaris with high activity against gram
positive compare with watery extract. MIC and MBC were measured and the results were
identical for the previous results, for the effect of both extracts on the growth of gram negative
and positive bacteria HPLC analysis was don for Berberis vulagaris which is quantitative and
qualitative assay to measure the concentration of some alkaloid compound Tetrandrine,
Chondocurine, bamine, which be with high ratio on Tetrandrine compound 38.42, the rate of
alkaloid compound in fruit of Berberis vulagaris was higher