Cytotoxic Effect of Gliotoxin, Hemolysin, Protease and Melanin purified from Aspergillus fumigates on REF Cell Line, in vitro Study

Abstract

Aspergillus fumigates especially clinical isolate produces a series of toxic substances and
proteinaceous hemolysin, protease and pigment like melanin which appear to act in an additive
and synergic way on cells. In this study, gliotoxin, hemolysin, Protease, and Melanin were used in
an experimental model to study their Cytotoxic activity by evaluating their effect on REF cell line
(Rat embryonic fibroblast ), for exposure time of 24 hrs at three different concentrations of
each compounds triplicate of each concentration were used, cytotoxicity of the purified
compounds were active against REF cell line under study and a toxic effect was clear with a
significant difference at the level of probability (p≤ 0.05) and this effect was contrasted among
different concentrations for each purified compound growth inhibition of REF cell line was
increase gradually with the increase of compound concentration.