Targeting Mice Mammary Adenocarcenoma Cells with Zinc Oxide Nanoparticles

Abstract

The cytotoxic effect of zinc oxide nanoparticles against mice mammary adenocarcenoma cells was
carried out in vitro. The used nanoparticles were synthesized by pulsed laser ablation in liquid
methods; the used liquid was deionized water. Colloidal suspension of zinc oxide nanoparticles was
synthesized by Q-switched Nd: YAG pulse laser device (1064 nm) laser energy was 600 mJ and pulse
duration was 10 nanosecond. High purity metallic zinc plate was submerged in 10 ml deionized
water in 50 ml glass baker and ablated with 1000 pulse at room temperature. The nanospecificities
of the synthesized nanoparticle colloidal were characterized with UV-Vis scanning
spectrophotometer. Atomic force microscope was used to determine the particle size distribution and
particles shape. The ZnO crestless formation was characterized by X-ray diffraction.
Mice mammary adenocarcenoma cells was treated with 25, 50, 100 ppm of ZnO nanoparticles.
Results indicated a significant toxicity of the ZnO nanoparticles toward cancer cells, this toxicity
correlated directly with ZnO nanoparticles elevated concentrations. These result need to be further
investigated and the molecular mechanism of ZnO nanoparticles effect should be more clarify.