Association Between PTPN22 Gene Polymorphism and Type1 Diabetes in Egyptian Population

Authors

  • Ali Salim Al-shehmany Al-shehmany
  • Ahmad A. El- Kafoury El- Kafoury
  • Medhat A. Haroun Haroun
  • Amira M. Embaby Embaby

DOI:

https://doi.org/10.24126/jobrc.2015.9.2.430

Keywords:

Type 1 diabetes, protein tyrosine phosphatase, nonreceptor 22, polymorphism

Abstract

The protein tyrosine phosphatase, nonreceptor 22 gene (PTPN22) maps at human chromosome 1p13.3
which encodes an important negative regulator of T-cell activation, lymphoid-specific phosphatase
(Lyp). The PTPN22 gene has been shown to associate with a risk for multiple autoimmune diseases,
including type1 diabetes (T1DM). This study aimed to analyze the association of three PTPN22
polymorphisms in Egyptian population .The single nucleotide polymorphisms (SNP) at positions -1123
(rs#2488457), 1858 (rs#2476601), and +2740 (rs#1217412) were genotyped in 60 persons with T1DM,
and 60 control persons, all three SNPs were genotyping using two technique, allele specific PCR
technique and restriction fragment length polymorphism – PCR (RFLP-PCR). The 1858 C/T did not
show any significance differences between patients and control groups. (
MCP=1.0) whereas, respectively
-1123 G/C and +2740 A/G were significantly associated with T1DM disease (P≤0.0001) and
(P=0.012).These results suggest that the PTPN22 gene of SNPs polymorphisms were associated with
type 1 diabetes in Egyptian population. The difference in the association of the aforementioned SNPs
variants with T1DM among different populations may be attributed to the presence of multiple
susceptibility alleles.

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Published

2015-06-01

How to Cite

Al-shehmany, A. S. A.- shehmany, El- Kafoury, A. A. E.-. K., Haroun, M. A. H., & Embaby, A. M. E. (2015). Association Between PTPN22 Gene Polymorphism and Type1 Diabetes in Egyptian Population. Journal of Biotechnology Research Center (JOBRC), 9(2), 29–36. https://doi.org/10.24126/jobrc.2015.9.2.430

Issue

Section

Research articles