Increasing the Production of Spilanthol in Spilanthes acmella (L.)Murr. by Using Tissue Culture Techniques

Abstract

This study was aimed to increase the production of Spilanthol from S. acmella by using tissue cultures.
Seeds were sterilized and cultured on MS medium supplemented with different concentrations 2.0,4.0,6.0
mg/l of Naphthalene acetic acid (NAA),1.0 , 2.0 , 3.0 mg/l Benzyl adenine (BA)and 1.0, 2.0, 3.0 mg/l Benzyl
amino purine (BAP). These growth regulaters were used for callus induction from leaf segments, plus 0.1
gm casein hydrolysate in combination with the same growth regulaters for callus induction and Spilanthol
production. Quality and quantity of Spilanthol were investingated using high performance liquid
chromatography (HPLC), results showed that using 1.5% sodium hypochlorate for 15 min was very
effective for disinfecting and survival . The combination of 3.0 mg/ l BAP, 6.0 mg/l NAA with 0.1 gm casein
hydrolysed was most effective for callus induction from leaf segments ,The highest mean fresh weight and
the highest amount of Spilanthol reached to 80.46 mg/g in combination of 2.0 mg/lBAP, 2.0 mg/l NAA with
0.1 g casein hydrolysed. This increased the Spilanthol compound to its highest amount when compaired
with the original plant.