PCR Detection of Some ESBLs (bla) Genes in Pseudomonas aeruginosa Isolated from Burn’s Units in Bagdad Hospitals

Authors

  • Asmaa A. . AL-Kaisse
  • Amina N. AL-Thwani
  • Rabab Q. AL-Segar

DOI:

https://doi.org/10.24126/jobrc.2015.9.2.439

Keywords:

Burn’s units, Pseudomonas aeruginosa, PCR, Resistance genes

Abstract

Multi drug resistant (MDR) and Extended Spectrum Beta Lactamase (ESBLs) of Pseudomonas aeruginosa
were detected. Pseudomonas aeruginosa is a bacterium responsible for severe infections in burn’s units,
plasmid DNA analysis and encoded many types of genes responsible for beta-lactamases. To determine the
type of genes responsible for beta-lactam broad spectrum in P. aeruginosa strains isolated from 100 swabs
of burn’s units environment, using a molecular methods (PCR) by primers specific to ESBLs (bla ) genes
oxacillin hydrolyzing capabilities OXA-10, OXA-4 and Vietnam Extended-Spectrum β-Lactame VEB-1.
The results revealed that 15 strains were isolated from burn units environment. All of 15 (100%) were
positive OXA-10 and only one (6.6%) for OXA-4 while the other gene VEB-1 was found in 6 (40%) isolates.تم 

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Published

2015-06-01

How to Cite

. AL-Kaisse, A. A. ., AL-Thwani, A. N. ., & AL-Segar, R. Q. . (2015). PCR Detection of Some ESBLs (bla) Genes in Pseudomonas aeruginosa Isolated from Burn’s Units in Bagdad Hospitals. Journal of Biotechnology Research Center, 9(2), 74–80. https://doi.org/10.24126/jobrc.2015.9.2.439

Issue

Section

Research articles