PCR Detection of Some ESBLs (bla) Genes in Pseudomonas aeruginosa Isolated from Burn’s Units in Bagdad Hospitals

Authors

  • Asmaa A. . AL-Kaisse
  • Amina N. AL-Thwani
  • Rabab Q. AL-Segar

DOI:

https://doi.org/10.24126/jobrc.2015.9.2.439

Keywords:

Burn’s units, Pseudomonas aeruginosa, PCR, Resistance genes

Abstract

Multi drug resistant (MDR) and Extended Spectrum Beta Lactamase (ESBLs) of Pseudomonas aeruginosa
were detected. Pseudomonas aeruginosa is a bacterium responsible for severe infections in burn’s units,
plasmid DNA analysis and encoded many types of genes responsible for beta-lactamases. To determine the
type of genes responsible for beta-lactam broad spectrum in P. aeruginosa strains isolated from 100 swabs
of burn’s units environment, using a molecular methods (PCR) by primers specific to ESBLs (bla ) genes
oxacillin hydrolyzing capabilities OXA-10, OXA-4 and Vietnam Extended-Spectrum β-Lactame VEB-1.
The results revealed that 15 strains were isolated from burn units environment. All of 15 (100%) were
positive OXA-10 and only one (6.6%) for OXA-4 while the other gene VEB-1 was found in 6 (40%) isolates.تم 

Downloads

Published

2015-06-01

How to Cite

. AL-Kaisse, A. A. ., AL-Thwani, A. N. ., & AL-Segar, R. Q. . (2015). PCR Detection of Some ESBLs (bla) Genes in Pseudomonas aeruginosa Isolated from Burn’s Units in Bagdad Hospitals. Journal of Biotechnology Research Center (JOBRC), 9(2), 74–80. https://doi.org/10.24126/jobrc.2015.9.2.439

Issue

Vol. 9 No. 2 (2015): Issues 2

Section

Research articles

License

This is an Open Access article distributed under the terms of the creative commons Attribution (CC BY) 4.0 license which permits unrestricted use, distribution, and reproduction in any medium or format, and to alter, transform, or build upon the material, including for commercial use, providing the original author is credited.