Detection the Ability of Aspergillus flavus Isolated from Wheat Grains for Aflatoxin B1 Production using RT- PCR

Authors

  • Eman Noaman Ismail

DOI:

https://doi.org/10.24126/jobrc.2015.9.2.443

Keywords:

AflatoxinsB1, Aspergillus flavus, RT–PCR

Abstract

Aspergillus flavus is aflatoxinogenic and potential aflatoxins producers in agricultural
commodities. The present study was conducted determine the ability of eleven strains of A. flavus
isolated from Iraqi wheat grains Triticum aestivum. The isolates have been detected by molecular
methods using Reverse Transcriptase RTPCR. In this study, RNA was extracted from A. flavus,
cDNA synthesis and rapid assessment of eleven isolates of A. flavus was accomplished using
primer pair for the aflatoxin regulatory gene aflR Reverse transcription-Polymerase chain
reaction (RT–PCR).Positive amplification was achieved for all the isolates with a molecular
weight 798 to aflR1 and 400bp to aflR2. Also the result of the amplification showed there are no
differences with the two molecular weight between the 11 isolated strains of A. flavus in their
aflatoxin B1 production, but the first strain differed in their banding florescence as compared
with others strains this reflect the genetic differences in aflatoxin B1 production between them.

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Published

2015-06-01

How to Cite

Ismail, E. N. . (2015). Detection the Ability of Aspergillus flavus Isolated from Wheat Grains for Aflatoxin B1 Production using RT- PCR. Journal of Biotechnology Research Center, 9(2), 102–106. https://doi.org/10.24126/jobrc.2015.9.2.443

Issue

Vol. 9 No. 2 (2015): Issues 2

Section

Research articles

License

This is an Open Access article distributed under the terms of the creative commons Attribution (CC BY) 4.0 license which permits unrestricted use, distribution, and reproduction in any medium or format, and to alter, transform, or build upon the material, including for commercial use, providing the original author is credited.