Polymorphism of The Promote Region of Chicken Insulin like Growth Factor-1 and Association With Bone Abnormalitiesالتغ

Abstract

The insulin like growth factor-1(IGF-1) is important regulators in stimulating growth, protein
synthesis, cell proliferation and differentiation in a variety of cell types. To achieve this goal,
blood samples were collected from 50 Gallus gallus affected from bone abnormalities (distortion)
and 50 samples a control group from the Animal farm/ College of Veterinary medicine/
University of Baghdad. DNA was isolated and the IGF-1genewas amplified by using specific
primers for promoter region and 5' UTR of this genes, then DNA sequencing was performed by
using AB13730XL, (Applied Bio system, Macro Gen company, USA). The DNA sequencing
results of flank sense of IGF-1gene from a control group was found to be compatible 100% with
wild type of Gallus gallus from the Gene Bank. On the other hand, sequencing results of the same
gene from 35cases abnormalities (distortion) revealed of 99% compatible and score 1351 and
expect 0.0 with the wild type sequences of gene bank. The minor differences could be attributed
to two transversion substitutions of (G>T,T>G) and (C>T) on locations 404, 104, and 249 nucleic
acid respectability. Fifteen cases of bone abnormalities (Distortion) of Gallus gallus, 99%
compatibility have one Transversion substitution G>T on position 404 andone transition
substitution (C>T) on 249 positions, under number Sequence ID: gb|JX414253.1|. In conclusion,
our case study suggests that polymorphisms of IGF-1gene are strongly associated with vertebrate
growth and development of some chicken breeds in Iraq. The aim of this study is to identify the
genetic polymorphisms of IGF-1gene and its association with vertebrate growth and development
of some chicken breeds in Iraq.