Detection of Helicobacter pylori IgG and IgM Antibodies in Iraqi Dyspeptic Patients

Abstract

This study is conducted to detect H. pylori IgG and IgM antibodies by different serological
techniques (Immunochromatography test for rapid H. pylori IgG antibodies detection and
enzyme linked immunosorbant assay test for H. pylori IgM antibodies detection). One hundred
and twenty five dyspeptic patients are subjected to esophageal gastroduodenoscopy at the
Endoscopy Unit at Baghdad Teaching Hospital from 10/1/2013 to 8/1/2014. Venous blood
samples are collected from each patient. This study recorded, (89%) sensitivity for each of IgM
Test by ELISA kit and Rapid IgG Test by Immunochromatography kit, but Rapid IgG Test has
more specificity (85.64%) than IgM Test (80.8%).The higher prevalence 31.2% of H. pylori IgM
antibodies observed among age group (21-40) years, whereas the IgM showed a slight rise 2.4%
from the (10-20) years age group as acute H. pylori infection, although statistically there is a nonsignificant (P ≥0.05) association between H. pylori IgM antibodies and gender, but the females
(32%) were more affected than males (27.2%). Also recorded significant (P ≤0.05) association
between chronic H. pylori infection and gender (male 45% and female 55%), and highly
significant association of blood groups phenotypes and Rh factor with H. pylori infection
recorded by rapid IgG test (P ≤0.01), as the seropositivity of H. pylori according to rapid IgG test
was (35, 15, 0 and 50) % for (A, B, AB and O) patients respectively, and (85and 15) % for Rh+
and Rh- patients, respectively.