Purification and Identification of Flavonoids Extracted from Loranthus Eurpaeus Fruits

Abstract

Loranthus eurpaeusis an important medicinal plant, which contains a lot of bioactive compounds. The dried plant
fruits were extracted overnight with 80% methanol by maceration using shaker incubator 25c◦
. Chemical
detection of crud plant extracts was performed. The total flavonoids was isolated from the extract using reflux,
and subjected to thin layer chromatography (TLC) using different mobile systems. The purified material was
augmented by using high performance liquid chromatography (HPLC). The aglycon moiety was extracted by
ethyl acetate, and then evaporated to dryness. The dried residue then redissolved in 50% ethanol. Purification
procedures of flavonoid were fully described in this study. The aims of this study was to detect the active
compounds present in L. eurpaeus methanolic extract then quantitative and qualitative estimation of the total
flavonoids isolated from the plant fruits.