Identification of Pseudomonas aeruginosa From Clinical Specimen by Using 16S rDNA Gene.

Abstract

Seventy- Five isolates of Pseudomonas aeruginosa were isolated from 100 isolates of clinical cases. 16S rDNA gene was detected by using polymerase chain reaction (PCR) and found that all isolates possess this gene, with 956 base pair. The isolates showed different sensitivity against antibiotics, all isolates were resistant to Kanamycin 100% most of isolate shown resistance to Ceftazidime 81.33%, Gentamicin 46.66 %, Tobramycin 38.66% and Piperacillin and Ofloxacin 37.33% each of them, and some isolates showed less resistance to Ciprofloxacin and Norfloxacin 34.66% each of them, and Aztreonam 22.66% and finally to Imipenem 17.33%. Virulence factors analysis showed 75 (100%) isolates were produced β-hemolytic, 61 (81.33%) were Protease activity, and 59 (78.66%) isolates had Pyocyanin and Amylase, and 54 (72%) isolates showed its ability to produce Biofilm.