Evaluation Efficiency of Pseudomonas fluorescens and Trichoderma viridi in Biological control of Rhizoctonia solani and Macrophomina phaseolina in Sesame Plant under Laboratory and Green House Conditions

Abstract

This study was conducted to evaluate the efficacy of biological control agents the fungus Trichoderma viridi (Tv) and the bacteria pseudomonas fluorescens (Pf) against two pathogenic fungi Rhizoctonia solani and Macrophomina phaseolina in Sesame plants under the laboratory and the glass house conditions. The pathogenicity test of both pathogen fungi isolates on sesame seedlings were as follow, the Rs-1 for R.solani gave incidence percentage of 100% and 80% and isolate Mp-8 for M.phaseolina gave 100% and 90% infection under laboratory and green house conditions, respectively. In antagonistic test the Tv-4 isolate gave antagonistic degree 1.2 and 1.3 against the isolate Rs-1 and Mp-8 , respectively . The Pf-6 isolate caused complete inhibition of both pathogen fungi .The study showed superiority of both bio control agents in reducing  of incidence percentage from 80% in infested control to 40 and 45 % in Pf and Tv  treatments , respectively , after 60 days of planting . These results caused highly increasing in number of pods (3.5 pods / plant ) for each of both bio control agents compared with infested control ( 1.8 pods / plant ). Bio control agents treatments has led to reduce the density of the fungi nursing in their treatment.