Isolation and Identification the Fungus Trichophyton violaceum from Human Skin Specimens in Iraq and Study Efficiency Antibacterial and some Plant Essential Oils

Abstract

This study was aimed to isolate and identify the fungus  Trichophyton  violaceum  from human  skin  specimens  and evaluate the  activity of  antibacterial  and  plant  essential oils. The results showed of direct microscopy by using KOH examination and morphological identification, nine samples from skin were positive in KOH test. Microscopical examination appeared that colonies of T. violaceum were coarse with abundant aerial mycelium on SDA, growth rates 6-8cm/14 days.  The color changes with age from white in the middle to brownish yellow at the edges which was more intense on the reverse side.  Susceptibility test to antibacterial showed that 100% of T.Violaceum T2 isolate was resistant to TE (Tetracycline), AK (Amikacin), CTX (Cefotaxime), CFM (Cefixime), CIP (Ciprofloxacin), ERY (Erythromycin) and STR (Streptomycin). Also results showed that all plant essential oils at different concentrations significantly inhibit growth of T. violaceum T2. However,  the  peppermint  oil,  Myrrh  oil,   Cardamom oil,  Chamomile oil  and  Castor oil  at  highest concentration (25mg / ml) caused highest reduction of mycelia growth (100%) followed by Olive oil (67.3%)  and Clove oil (64%) at the same  concentration,  compared with the control  treatment.