Study the Effect of Optimum Conditions for L-Asparaginase Production from Local Isolate of Erwinia caratovora AH88

Abstract

The aim of this study was to determine the optimum conditions for L-asparaginase production form local isolate Erwinia caratovora AH88 (gained from previous study) by using submerged culture. The results  of this study revealed   that the optimum conditions for L-asparaginase production from this isolate were include using1 % of lactose  as carbon source, 1% yeast extract as nitrogen source,  in addition to asparagine with 0.19 % as inducer. The optimum pH was 7, optimum temperature was 30 C^o, aeration speed was 150 rpm, optimum size of inoculum was 2% (contain 3 X 10⁶    CFU/ ml) and incubation period was 24 hours.  Under these optimum conditions for L- asparaginase production the results revealed that the enzyme activity and specific activity were 71.0 unit / ml and 17 unit / mg respectively.