Qualitative and Quantitative Estimation of Flavonoids Extracted from Caraway (Carum carvi L.) Seeds

Abstract

Caraway (Carum carvi L.) was an important medicinal plant, which contains a lot of secondary metabolites with wide bioactivity. The dried seeds plant was extracted overnight with four solvents separately: 70% methanol, 70% ethanol, 70% n-hexane and water by continuous hot extraction using Soxhlet apparatus for the three solvents and by maceration with the last. Chemical detection for major active constituents in the four extracts was performed. The flavonoids was isolated from the crud plant using reflux, and subjected to thin layer chromatography (TLC). The aglycon moiety was extracted with chloroform.The extracted material was augmented by using high performance liquid chromatography (HPLC).The result of this study was indicated that each solvent extracts different compounds than the other and the plant was rich with flavonoids specially Rutin, Quarecetin kaempferol, Luteolin and less amount of Cumarins. The total flavonoid in each one gram dried caraway seed is 0.78mg.