Detection of Polymorphism in the Gene blaKPC2 of Local Klebsiella pneumoniae Isolated from Iraqi Patients

Abstract

Carbapenemases are clinically important because they destroy and may confer a resistance to carbapenems, severe infections caused by carbapenemase producers is associated with increased mortality. To achieve this goal, 180 samples were collected from different clinical sources included 92 urine, 33 smears of wounds, 13 smears of burns and 42 sputum. The samples were taken from patients attended Al-Yarmouk Teaching Hospital and Ibin Baladi Hospital in Baghdad Governorate. Diagnosis of bacterial isolates was done depending upon the microscopic examination, the cultured characteristics and biochemical tests. DNA extracted from 84 samples. Accordingly, detection of bla_KPC2 gene was conducted by using specific primers for amplification of bla_KPC2 gene. Moreover, the sequencing of 910 bp for bla_KPC2 gene was performed by the biotechnology lab. at the National Instrumentation Center for Environmental Management (NICEM). Such test has been implanted by using 3730XL as a DNA sequences. The obtained results were analyzed by blast at the National Center Biotechnology Information (NCBI) and detect polymorphism in bla_KPC2 based on the Bio Edit. Consequently, 94 variations between 47 transversions, 43 transitions and 4 deletions nucleotide were noticed. In a sense the test showed 79% under sequence ID gb|CP009872.1| from 3037673 -3038166 number of nucleotide from K. pneumoniae subsp. pneumoniae strain KPNIH30 of Gene Bank, score (329) and expect 5e-86 with the wild type of bla_KPC2 gene from Gene Bank. Finally, the results illustrated polymorphism between local strains of