Isolation and Identification of the Bacteria which Contaminated the Tigris River in Some Districts of Wasit Province

Abstract

The bacteriological study was included isolation and identification aquatic bacteria of dominant species in Tigris River. The period of the study for one year which starting from June 2015 until 2016 May which include 8 stations which distributed as follows : two station in the each site with space 50-meter between them, which have all of the following districts counties run through AL-Azizia AL- Zubaydiah, AL-Numaniyah and AL-Akut distributed as two repeaters within Wasit province. Identifications methods using the biochemical tests and verify the identification by API20E system. Results have pointed to the appearance of the isolation of 22 belonging to the following genera and species in the river water samples involve: Aeromnonas veronii , Aeromonas punctata , Bacillus subtilis , Exiguobacterium profoun , Pseudomonas alcaligenes , Enterobacter cloacae , Aeromonas caviae , Pseudomonas fluroescen , Bordetella spp , Photobacterium damala , Aeromonas hydrophila , Acinetobacter bumannii , Serratia liquefciens , Moraxella spp , Buttiaxella agrestis , Shigella spp , Chryseomonas meningosept , Pasteurella spp , Erwinia spp , Escherichia coli , Enterobacterium hormaechei ,  and Escherichia vuleneris. Temporal variation of aquatic bacteria in the study area was include:  16 bacterial isolate at June 2015 (27.5 %) 16 isolate at September (27.5%), 12 isolate at December (20.6 %) and 14 isolate in the May 2016 (24.1%). This study discover that station 1 was the most contaminated because domestic sewage and cattle feces within agricultural activities.