Optimization Condition of Chitosan Produced by Submerged Fermentation from Aspergillus oryzae SU-B2

Abstract

Chitosan, the deacetylated form of chitin, is a polysaccharide formed primarily of repeating units of D-glucosamine. It is a versatile biopolymer exhibiting various unique biological properties hence finds wide applications in food, biomedical and chemical industries. This study was conducted to evaluate optimum condition for chitosan production from Aspergillus oryzae SU-B2. The result have showed the best concentration 2% glucose as carbon sources, while 0.5 %(NH₄)₂SO₄ and 0.1% yeast extract were optimum concentration as nitrogen sources. The optimum pH was 5.5 whereas the optimum temperature is 30˚C with incubation time 120h and agitation rate 150 rpm. The high yield of chitosan 10200 mg/ml was obtained with average increase 207.2%.