Effect of Nanoparticles on in vitro Microtuberization of Potato Cultivars (Solanum tuberosum L.)

Abstract

Study the effect of two nanoparticles compounds namely Silver nitrate nanoparticle (AgNO₃-NPs) and Titanium dioxide nanoparticle (TiO₂-NPs) was used in MS medium on plantlets growth and microtuberization of three potato cultivars (Arizona, Riviera and Burren) in vitro. The results showed significant differences between potato cultivars in the response of tissue culture and microtubers production. Arizona cultivar gave highest length, fresh and dry weight of plantlets and number of micro tubers production. It done 13.61 cm/plantlet, 1283.48, 145.04 mg/plantlet and 2.83 micro tuber/plantlet respectively. Riviera cultivar gave a highest weight of microtuber/plantlet and Burren cultivar done a highest diameter of micro tuber and they significant compared with Arizona variety. The result also showed that 2 mg/l of AgNO₃-NPs caused significant increase in height, fresh and dry weight of plantlet, weight and diameter of micro tuber, 15.05 cm/plantlet, 1021.44, 13897 mg/plantlet, 317.35 mg/ micro tuber and 6.07 mm/micro tuber respectively. The 2mg/l of TiO₂-NPs gave a highest number of micro tuber achieved 2.48 micro tuber/plantlet; it was statistically significant compared with the control and 2mg/l of AgNO₃-NPs, as well as showed significant interactions between treatments and varieties in the most of characterization studies.