Use of sheep horn hydrolysate as nitrogen source for lactic acid bacteria growth

Abstract

This study included used the sheep horn Hydrolysate as a nitrogen source in growth medium (SHHM) for cultivation of lactic acid bacteria, The results showed the optical density of growth in 540 nm for Lactobacillus casei and lactobacillus acidophillus was 1.38 and 1.31 respectively after cultivation in (SHHM) medium. Otherwise the optical density of growth for Lactobacillus casei and lactobacillus acidophillus was 1.27 and 1.32, respectively after cultivation in (MRS) medium, Which contain peptone, yeast extract and meat extract as a nitrogen source. In the inhibition capability test the results showed that the inhibition zone average of cell free extract for lactobacillus casei which cultivated in SHHM was (14 , 13 and 18) mm against Klebsiella sp, Staphylococcus aureus and Candida sp. respectively, while the inhibition zone average of cell free extract for lactobacillus casei which cultivated in MRS was (12 , 10 and 12 mm) against Klebsiella sp, Staphylococcus aureus and Candida sp. Respectively. The inhibition zone average of cell free extract for lactobacillus acidophillus which cultivated in SHHM was (14 , 14 and 20) mm against Klebsiella sp, Staphylococcus aureus and Candida sp. respectively, and ( 13 , 13 and 16 ) mm against Klebsiella sp, Staphylococcus aureus and Candida sp. respectively after cultivated in MRS medium.