Extraction and Purification of the Urease Enzyme from the Proteus mirabilis Bacteria

Abstract

Bacterial isolate was identified using vitek diagnosis; it's obtained from burns from Al-imam Ali Hospital which proved its ability to produce Urease by using Luria broth culture media because it is suitable for growing bacterial cells and for extraction of enzyme. Enzyme purification processes include precipitation with Ammonium sulfate, ion exchange chromatography and gel filtration then the enzyme activity was measured for each purification step. The results of  Urease purification  that  extracted from the isolate of  Proteus mirabilis using ammonium sulphate showed that the specific  activity  was  4.71 unit/mg, and the best  saturation ratio was (60%), the number of purification times and  yield were 3.5and14.6%  respectively. Furthermore, the result of purification using  Ionic exchange - Cellulose DEAE was gave the specific   activity 12.29 units/mg, the number of purification times  and  the  enzymatic yield were 9.2, 8% respectively while the specific  activity  was 15.1units/mg  and the number of purification times and the enzymatic yield 11.27and 14% respectively by using gel filtration.