Cytotoxic activity of Cardia draba leaves extracted on some cancer cell line

Abstract

        Cardaria draba is considered as one of the most fundamental plants that have wide diversity in medical uses, other researches had shown that this plant has a strong antioxidant activity for this reason the idea came to look for the cytotoxicity of this plant extract against different carcinogenic cell lines after subjection of this plant to methanolic extraction method.Result declared strong cytotoxicity against those carcinogenic cell line with a variable  values of IC₅₀ and the best effect was seen against HepG2 cell line, then we looked for the reason behind this reduction in viability of the cells via performing Caspase 9 assay which is used to check the induction of apoptosis and the expression of the TNF-α as a strong transcription factor which is activated in tumor cell lines.

        Interestingly, our finding suggested that the plant extract produced a positive effect in the induction of Caspase 9 which led to induce early apoptosis, regarding TNF-α expression; the result explained a significant reduction in expression of those genes which were correlated with induction of tumor. This meant that the reduction in cell viability resulted in the induction of apoptosis and reduction of TNF-α transcription genes.