The effect of cell-free supernatant (CFS) of Escherichia coli on human sperm motility

Abstract

         Escherichia coli were isolated from vaginal and cervical samples during vaginal examination. Fifteen semen samples have been gathered through masturbation after three days of sexual abstinence. The Cell-Free Supernatant (CFS) of Escherichia has been collected by removing the cells from overnight broth cultures and filtered. The effect of (CFS) of Escherichia coli on spermatozoa motility was determined by using Earl’s balanced solution to prepare1:2 to 1:16 dilutions of CSF. Results conducted there was a significant difference in the count number of motile spermatozoa in 15 seminal fluid samples after  being treated with CSF at the dilutions1:2 (p≤0.05) ,1:4 (p≤0.05) and 1:8(p≤0.05). The experiment showed a successive significant  increase  in the count number of motile sperms compatible with the lowering concentration of CSF until the count number of  motile sperms reached the highest in the fourth dilution 1:16 ( P≥0.05)  . This demonstrates the real effect of CSF on the vitality and activity of sperm. On the other hand, the study showed that the Escherichia Coli, causing genital tract infection, could have a bad effect on the motility and transportation of men’s spermatozoa and might be responsible for infertility.