RAPD and ISSR analyses of Saccharomyces cerevisiae isolates from different sources

Authors

  • Sawas Younus Hammadi
  • Anmar Sael Hussein
  • Duha Mysire Majeed
  • Batol I. Dheeb
  • Eman Noaman Ismail

DOI:

https://doi.org/10.24126/jobrc.2018.12.2.537

Keywords:

RAPD and ISSR assay, Saccharomyces cerevisiae, Bread yeast, Apple, Plum, Dates, Peach

Abstract

     The purpose of this study was to isolate the Saccharomyces cerevisiae present on different fruits and performing RAPD and ISSR analyses to know the genetic interrelationship between different S. cerevisiae isolates. Some fruits namely apple, plum, dates, and peach were used as natural sources for S. cerevisiae isolation. The isolated S. cerevisiae was designated as SUC1, SUC2, SUC3, SUC4, SUC5 respectively. Amplicon fingerprints for the isolated species were obtained by RAPD assay using six different primers and ISSR assay using six different primers. RAPD assay showed the lowest genetic distance (0.1559) between SUC2 and SUC3 isolates whereas ISSR assay showed the lowest genetic distance (0.06899) between SUC4 and SUC5 isolates.  Both genetic markers showed the highest genetic distance for SUC1 when compared to the other isolates.

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Published

2018-06-01

How to Cite

Hammadi, S. Y. . ., Hussein, A. S. ., Majeed, D. M. ., Dheeb, B. . I. ., & Ismail, E. N. . (2018). RAPD and ISSR analyses of Saccharomyces cerevisiae isolates from different sources. Journal of Biotechnology Research Center, 12(2), 40–50. https://doi.org/10.24126/jobrc.2018.12.2.537

Issue

Vol. 12 No. 2 (2018): Issues 2

Section

Research articles

License

This is an Open Access article distributed under the terms of the creative commons Attribution (CC BY) 4.0 license which permits unrestricted use, distribution, and reproduction in any medium or format, and to alter, transform, or build upon the material, including for commercial use, providing the original author is credited.