The activity of extracts chara vulgaris against promastigotes of leishmania tropica.

Abstract

            Leishmaniasis is a widespread parasitic disease caused by Leishmania parasite, this disease considers as a major health problem worldwide. The available therapy is unsatisfactory expensive with a cytotoxic side effects. Studies of marine algae as a source of pharmacological active compounds have increased worldwide.  This study was aimed to investigate the effect of a type of green algae (Chara vulgaris) on promastigotes of L. tropica, by using. various concentrations (500, 250, 125, 62.5, 31.25, 15.6 µg/mL) in vitro by MTT assay [3-(4.5-dimethylthiazol-2-yl)- 2.5-diphenyl tetrazolium bromide)], to investigate its effect on the proliferation of promastigotes, by three incubation periods (24, 48, 72 hr.) The results showed a significant (p< 0.05)   decrease in survived of promastigotes in treatment groups with concentrations that ranged between 15 to 500 μg/ ml.  This study revealed a major growth inhibition effect of the organic extract of C. vulgaris against L. tropica promastigotes, and the extract of ethyl acetate showed potential activity is better than the aqueous extract.